General
Learning outcomes and competences
The student will have a laboratory hands on learning process of the methodologies available for producing experimental models, their fundaments, applications, practical requirements, advantages, limitations, and the type of answers they can provide. The student will be confronted with questioning the adequacy of the models to the objective of the study and their correct validation at the molecular, biochemical and behavioral levels. The student will be able to plan the best approach to scientific questions in different neuroscience fields by using the most appropriate experimental model. These competencies will help the student with the design and execution of the master thesis’s research project and will allow him to understand the work performed by others, leading him to seek collaborations outside their walls as a way of getting complementary data.Program
The curricular unit is organized in four modules that complement each other allowing students have contact with the theoretical and practical fundaments concerning the production of different types of in vivo (surgical, metabolic, inflammatory), in vitro (cell culture), mutants and transgenic experimental models useful in the investigation of the various areas of neuroscience. Their advantages and disadvantages will be discussed, as well as the principles that should govern the choice of the most appropriate model for the objectives of each study and the adequate biochemical, molecular and behavioral validation of the experimental model.
Module 1 – Disease models in wild-type animals (production and validation) – Fani Neto (coordinator): Principles for production and validation of animal models; Models of degenerative diseases, Models of somatic chronic pain, Models of visceral inflammation and pain, Models of nervous system injury, Animal models of addition, Zebra fish models in neuroscience research, The use of Drophosila in neuroscience research, Models of metabolic diseases, Validation of animal models.Module 2 – Genetic Manipulation in Mice: Mutation and Transgenesis – Miguel Soares (coordinator): Mouse development; harvest, culture and manipulation of preimplantation-stage embryos; Production of transgenic and chimeric mice; Strategies for embryonic stem cell-based transgenesis; genome alterations and introduction of foreign DNA into ES cells; Detection and analysis of mouse genome.
Module 3 – In vitro models – Adriana Rodrigues (coordinator): Introduction to neuronal cell cultures, Introductory training for Cell Culture Basics, Primary cell culture, Neuronal cell lines, Organotypic cultures, Ex-vivo culture of rodent hippocampus
Module 4 – Behavioural analysis – Joana Ferreira Gomes & Clara Monteiro (coordinators): Ethological analysis of behavior, Rodent behavior - Psychophysical assessment, Rodent behavior - Cognitive assessment, Planning experiments of behavior in neuroscience, Practical analysis of behavior.Eligibility for exams
Frequency in the four modules with minimal approval in each;Calculation formula of final grade
The final grade is the average of the marks obtained in each module.
(M1+M2+M3+M4)/4
The evaluation in each module will take into account the student’s critical abilities and previous preparation for each session, and his performance at the practical sessions and will be as follows:
Module 1: individual written work about an in vivo disease model in neuroscience with a critical view of the pros and cons;
Module 2: Selection and oral presentation of a research paper using of a genetically manipulated mouse model and critical evaluation of the adequacy of the model to the scientific questions;
Module 3: Individual written work adapted to the module's theme;
Module 4: Presentation and discussion of articles by students.Classification improvement
It is possible to improve the classification obtained in the curricular unit, during the extra exams season, by doing a global exam which includes all the content lectured in each of the individual modules that constitute the curricular unit. It is also allowed to improve the classification by attending again each module separatelly in a new year ("Melhoria por Frequência").- FINAL GRADES IN THE UC File Uploaded 13/07/23, 23:47